Phosphate buffer was used for experiments at pH 7.0 and 8.0, and acetic acid buffer was used for those at pH 4.3 and 5.3.
Sodium acetate buffer was used for pH 4 6; Sodium-phosphate buffer was used for pH from 6 to 7 and Tris HCl buffer for pH 7 10.
Agarose 0.8%% (w/v) in 0.5X TAE (pH 8.0) buffer was used for submarine gel electrophoresis.
After that, FDA and PI working buffer was used for cell staining (Calcein AM/Ethidium Homodimer, Invitrogen).
Native alpha chymotrypsin (1 mg mL-1) solution in the aqueous buffer was used for recording the spectrum.
Figure 2 showed the real-time SPR binding curves in the injection of sample, while the PBS buffer was used for the SPR running buffer.
An aqueous solution of 0.1 M buffer was used for each measurement with 0.2 M sodium sulfate as a supporting electrolyte.
We showed improved labelling of DTPA- and DOTA-conjugated peptides, proteins and antibodies with 111In when HEPES or MES buffer was used for radiolabelling.
A 50 mM malonate buffer was used for elution at a flow rate of 0.3 mL/min and active fractions (up to 30, 1 mL) were collected and absorbance was measured at 280 nm (CE Cecil 7200, Germany).
The spectra were corrected for the background by subtracting the spectrum of the solvent, i.e. 50 mM sodium phosphate buffer, pH 7.8 for native alpha chymotrypsin and n-propanol for the EPRP and ECCN 3. 1 mg mL-1 native alpha chymotrypsin solution in the aqueous buffer was used for recording the spectrum.
This buffer was used for all experiments.
