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100 µL of extraction buffer was used as a control.
An aliquot of 250 500 ng of DNA in 100 µl TE buffer was used as input for library generation.
Acetate buffer was used as the blank.
PBS buffer was used as a negative control.
The TB medium without glycerol and PBS buffer was used as the control.
Hence, 30 mM phosphate buffer was used as the aqueous phase in this study.
0.1 M phosphate buffer was used as it gaves the highest number of theoretical plates with good resolution.
A 50ml polypropylene Falcon tube containing a slip of filter paper soaked in hybridization buffer was used as a hybridization chamber, as described by Stahl and Amann [1991].
Briefly, sodium orthovanadate (Na3VO4), verapamil, or test compounds diluted in assay buffer were added to a 96-well white opaque plate, while the assay buffer was used as negative control.
The second buffer was used as the basis of the software's undo feature.
O-phenylenediamine dihydrochloride (OPD) in citrate buffer was used as substrate for the peroxydase.
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