Sentence examples for buffer was thereafter added from inspiring English sources

Twenty microliters of hybridization buffer was thereafter added to the RCA mix to obtain the final batch of DNA-coils.

The plant extract (75 μl) was thereafter added.

Thereafter, 5 ul of Annexin V FITC and 10 ul of PI buffer were added to each tube before incubating in the dark for 15 min at RT. 400 ul of 1x Annexin V-binding buffer was then added to each tube and flow cytometric analysis carried out within one hour.

Solubilisation buffer was then added.

Thereafter, 150  μl solubilization buffer was added and cells were further incubated at 37 °C in the dark for 2 h.

Thereafter, 25 μl of dilution buffer was added and the material was ready for use in the qPCR.

Thereafter another 15 ml of disassembly buffer was added and mixed with the sonication liquid, the sample was centrifuged at 15,000 rpm for 15 min.

After the lysis buffer was added, the samples were placed on ice for 15 minutes and thereafter centrifuged for 30 minutes at 500 g.

Thereafter, 50 μL enzyme solution was added to all wells (except the negative control H12, to which 50 μL additional buffer was added instead) and the plate was pre-incubated for 5 min.

In short, cells (20000/well) were pre-incubated at normoxia, hypoxia or anoxia, where after drugs were added and the plates incubated for 72 hrs, washed ones with PBS in a microtiter plate washer (Multiwash, Dynatech Laboratories) and thereafter FDA (100 μl of 0.01 mg/mL FDA, Sigma-Aldrich, Stockholm, Sweden) in a buffer, was added.

Formamide loading buffer was added.