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The resultant buffer was then stored in the glovebox and used for all the thiol disulfide exchange experiments.
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The DNA was re-suspended by adding 50 μl of 0.1 M TE buffer and was then stored at –20°C until use.
The resulting buffer-equilibrated Triton X-114 was then stored below its cloud point at 4°C.
For analysis, cell pellets were resuspended in FACS buffer and were then stored at 4°C in the dark and were analyzed by flow cytometry within the next 2 h(Fig. 2).
Samples were processed by filtering 1 liter of seawater onto 0.2 µm Sterivex filters (Millipore, Billerica, MA) and flooding the 2 mL reservoir of the filter cartridge with Puregene Lysis buffer (Gentra Systems, Minneapolis, MN) which were then stored at −80°C until processing.
The samples were then stored with sodium cacodylate buffer (about pH 7) until further use.
The video data is then stored in a play-out buffer at the client side before being displayed.
Samples were then stored at − 20 °C in 0.1 M phosphate buffer prior to further testing.
Solubilisation buffer was then added.
Its buffer is then cleared.
The elution buffer containing fractionated and digested peptides was then removed and stored in a microcentrifuge tube until MALDI-TOF-MS analysis.
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