Sentence examples for buffer was then replaced from inspiring English sources

The buffer was then replaced with 0.5 ml of Krebs buffer containing 10  μCi 5-H-glucose in the presence of 10 mM cold glucose and the cells were incubated for 1 h at 37 °C.

Ringer's buffer was then replaced to osmolarity-corrected zinc fixative (Zn-fix) solution (4.5 mM CaCl2, 52 mM ZnCl2, 32 mM Zn(CF3COO 2, 2 mM Tris, 38 mM glycine; pH 6.5, 340 mOsm/l) [ 34].

The OGD buffer was then replaced by a conditioned medium and the cultures were returned to the humidified 95%air/5%% CO2 incubator for the indicated post-incubation time period.

The stop buffer was then replaced with a minimal volume of 2× SDS-PAGE buffer (62.5 mM Tris-HCl, pH 6.8, 10% glycerol, 2% SDS, 5% β-mercap-toethanol, 0.05% wt/vol bromophenol blue).

The buffer was then replaced by a proteolysis buffer containing 2.5 ml lysis buffer and 100 μl Proteinas K (from a stock solution of 50 U/ml; Roche Diagnostics Scandinavia AB, Bromma, Sweden) and the plugs were incubated for approximately 24 h at 56°C with gentle shaking.

The Tris tricine SDS buffer is then replaced with Tris glycine buffer (no SDS) in both wells for an additional 10 min washout at 100 V/cm in the same direction.

The liquid in the tubes was replaced twice with Assay buffer to remove bacteria and was then replaced with 400 mM ethanol in Assay buffer.

Compound solution flow was then replaced by buffer flow, resulting in dissociation of the complex.

The supernatant was then replaced with Ca2+ buffers containing higher concentration of calcium (150 mM).

The mix was then replaced with the stop buffer.

It was then replaced with fresh developing buffer and the gel was incubated at 37°C for 16 h.