Sentence examples for buffer was then removed from inspiring English sources

The buffer was then removed, and the slides were placed into staining jars containing radioligand solution (concentration range of 0.1 10 nM) in the incubation buffer with or without 10 μM raclopride (D2/3 antagonist) or 100 μM guanosine-5'-triphosphate sodium salt (GTP, stimulator of G-protein uncoupling from the receptors).

The lower running buffer was then removed and the lower chamber rinsed with nuclease-free water 2 times.

The lower running buffer was then removed and the RNA was purified using the flashPAGE™ clean up kit, producing RNA fragments ranging from ∼50 150 nt in size.

Excess RIPA buffer was then removed from the beads.

The SAPE/Amplification Buffer was then removed by vacuum filtration.

Dye loading buffer was then removed and replaced by pre-warmed complete growth media.

Somatic cells were eluted with 3 ml of MACS buffer and the column was then removed from the magnetic backing and germ cells were eluted in 3 ml of MACS buffer.

The elution buffer containing fractionated and digested peptides was then removed and stored in a microcentrifuge tube until MALDI-TOF-MS analysis.

The heart was then removed and minced in digestion buffer, to which stop buffer (perfusion buffer containing 12.5 µM CaCl2 and 5% newborn calf serum) was added.

The supernatant was then removed and sample buffer added to the beads.

The proteinase K was then removed and the buffer was exchanged using Microcon YM-100 column (Millipore Corporation, Bedford, USA) in four rounds of centrifugation at 500 g for 15 min at 23°C.