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To determine the off curve, the EMSA buffer was then pumped through the reaction chamber at a speed of 200 μl/min until the OIRD signals stabilized.
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The filtered lysate was then pumped into a 5 ml HisTrap column (GE Healthcare) and washed with ten column volumes of buffer A containing 40 mM imidazole.
It is then pumped back through a series of filters.
The products are then pumped out by the vacuum system.
Finally, the monomeric substrates are then pumped out in the cytosol for recycling purposes [ 25].
Two pore volumes of 70% ethanol were then pumped through the columns.
A two-goal buffer was then established after Catriona Wagg converted another chance.
Solubilisation buffer was then added.
Its buffer is then cleared.
This suspension was then directly pumped into our microfluidic device.
The first action in the on-chip RNA isolation protocol is the transport of diluted sample from the sample reservoir into the prefilled lysis buffer reservoir, which is then reciprocally pumped between compartments until the two fluids are homogenous.
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