Sentence examples for buffer was then plated from inspiring English sources

Serial dilution of the eluted bacteria in DE buffer was then plated onto TSA, and the plates were incubated for 18 24 h at 37°C.

Residual EMS was neutralized with 1 M NaOH and removed by two washes with 4 ml M9 buffer, and the worms were then plated on OP50-seeded NGM plates.

Streptavidin HRP (horseradish peroxidase; Pierce; 0.1 μg/ml) in the corresponding buffer was then added and plates were incubated for 30 min at room temperature, before being washed again.

Standard lysis buffer was then added to each plate and plates were incubated on ice.

A total of 1.5 ml of M9 buffer was then added to each plate and, using a glass spreader, the bacterial lawn was scraped from the plate.

Solubilisation buffer was then added.

Serum samples (diluted 1 in 110 in a blocking buffer) were then added to the plates and allowed to incubate at room temperature with shaking for 90 min. Following incubation, plates were washed and horseradish peroxidase (HRP -conjugated rabbit anti-HRP -conjugated, Glostrabbitenmark) was added.

Serum samples (diluted 1 in 110 in a blocking buffer) were then added to the plates and allowed to incubate at room temperature with shaking for 90 min. Following incubation, plates were washed and horseradish peroxidase-conjugated rabbit anti-human IgG (Dako, Glostrup, Denmark) was added.

The top agar containing the plaques and the second volume of SM buffer were then scraped from the agar plates and added to the former sample.

Serial dilutions of Rad51 in blocking buffer were then added to the appropriate wells of the ELISA plate and incubated for 1 h at 30°C.

Its buffer is then cleared.