Sentence examples for buffer was then perfused from inspiring English sources

Twenty microliters of Tyrode-HEPES buffer was then perfused though the system to remove unattached cells.

The heart was then perfused with 25 ml dissociation buffer containing 144 U/ml collagenase (Worthington type-2) and 50 µM CaCl2.

Soluble C8-PIP2 was then perfused with a microperfusion pipette aimed directly at the membrane to activate channels: during perfusion of C8-PIP2 channels opened and during perfusion of buffer they closed as soluble C8-PIP2 diffused away from the membrane.

The hearts were then perfused with perfusion buffer containing 0.3% collagenase for 45 minutes, minced and dissociated in incubation buffer (perfusion buffer with 0.2% BSA and 0.3 mM CaCl2) containing 0.3% collagenase.

Mice were then perfused (gravity perfusion) through the left ventricle for 15 minutes with either phosphate buffered saline (PBS) (minimum of four mice/experiment) or 4% paraformaldehyde (PFA) (minimum two mice/experiment).

Hearts were then perfused with buffer containing 0.2 mM palmitate pre-bound to essentially fatty acid free 1.5% wt/vol bovine serum albumin (BSA) for 20 min.

Cells were then perfused with KHM buffer containing 40 µM digitonin until ECFP fluorescence started to dissipate.

Cells were then perfused continuously with buffer containing stimulus or vehicle for 500 s.

All preparations were then perfused for 30 min each with perfusion buffer containing increasing concentrations of propranolol (0.1 and 1.0 μ m) [ Fig. 4 ii),(iii)].

To this end the animals received a weight-adjusted dose of pentobarbital (Nembutal; Sanofi Sante B.V., Maassluis, The Netherlands) and were then perfused with sterile phosphate buffered saline.

The rats were then perfused transcardially with 0.9% saline, followed by 500 ml of 4% paraformaldehyde in phosphate buffer fixative (0.1 M, pH 7.4).