Sentence examples for buffer was then filtered from inspiring English sources
0.1 M buffer was then filtered through a 0.22 μm sterile filter and degassed by sonication (30 min).
As at high concentrations (>1.2 mg ml 1) GS-168AT2 tonds to precipitate, it was stored under solution in buffer B. The purified protein was then filtered through 0.45 μm membranes and protein content was quantified by Bradford assay and by SDS PAGE coupled to gel slab analysis using Gene Genius system (Syngene, Cambridge, UK).
The mixture was then filtered and washed with 500 mL of coupling buffer, and the residue was divided into four parts.
The solution was then filtered through Whatman filter paper (0.45 μ) and diluted suitably with phosphate buffer saline pH 7.4.
The digested mixture/slurry was then filtered using a 70 μm filter followed by lysis of red blood cells using ammonium chloride/potassium buffer according to the manufacturer's protocol (Lonza).
The sample was then filtered using a 25 mm Swinnex® filter holder (Millipore) fitted with a double layer of 48 μM nylon filter and the final volume of the filtrate was adjusted to 700 μL with buffer.
Extract was then filtered with Whatman number 1 filter paper.
The mixture was then filtered through Whatman No.1 filter paper.
Extract was then filtered and freeze dried.
It was then filtered through coal bed.
The tCNSL was then filtered and stored.
