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The buffer was then exchanged with distilled water and dialysis was continued for 24 h.
HAP column buffer was then exchanged and washed extensively with buffer D at 0.03 M KPi, pH 6.8 without KCl and NP-40.
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The remaining buffer in the microcuvette was then exchanged 4 times that evening, and exchanged 3 times the following morning to assure that the glucose concentration was effectively zero.
The buffer was then fully exchanged with HBSS containing d-[H]aspartate (final concentration: 0.1 μCi/ml) and 50 μM of unlabelled aspartate as a carrier.
Pooled fractions were then exchanged to 150 mM NaCl, 10 mM Tris, and 2 mM EDTA, pH 7.4 buffer.
Valproylcarnitine is then exchanged for free carnitine by carnitine translocase.
The sample buffer was then desalted, and the construct was further purified by ion exchange as described for p6004480 5183.
The resultant buffer was then stored in the glovebox and used for all the thiol disulfide exchange experiments.
A two-goal buffer was then established after Catriona Wagg converted another chance.
Solubilisation buffer was then added.
Its buffer is then cleared.
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CEO of Professional Science Editing for Scientists @ prosciediting.com