Sentence examples for buffer was then drained from inspiring English sources

The buffer was then drained out from the ileal section, phenylmethylsulfonyl fluoride was added, and the suspension was centrifuged at 100 g for 3 min.

After washing the membrane with washing buffer 4 × 5 min, the excess buffer was then drained off and the membrane transferred into a clean container.

The slides were then drained and flooded slowly with three changes of neutralisation buffer (0.4  M Tris, pH 7.5) for 5 min each and then stained with 50  μl of ethidium bromide (20  μg ml−1) and covered with a coverslip for immediate analysis.

The samples were then drained and the beads were equilibrated in 3 mL coupling buffer before adding 1 mL 0.1 M sodium cyanoborohydride in coupling buffer and mixing overnight at 4 °C.

Slides were then drained, placed on a tray and flooded slowly with three changes of neutralisation buffer (0.4 Tris pH 7.5) each for 5 min, to remove alkali and detergents.

The slides were then drained, placed on a tray and flooded slowly with three changes of a neutralisation buffer (0.4  M Tris, pH 7.5), each for 5 min, to remove alkali.

The juice is then drained off and fermented separately.

The slides were then drained.

The wound is then drained and closed.

The water is then drained and the rice harvested.

The contents were then drained and collected before repeating this step for an additional 15 minutes.