Sentence examples for buffer was then discarded from inspiring English sources

Wash buffer was then discarded and replaced by 100 µl fresh WB supplemented with 50 units of benzonase (Sigma).

In-gel reduction/alkylation: 10 mM DTT in 100 mM ammonium bicarbonate was added to gel bands and incubated at 56°C for 60 mins, the buffer was then discarded.

Next, 50 mM IAA in 100 mM ammonium bicarbonate was added to gel bands and incubated at room temperature in the dark for 30 mins, the buffer was then discarded.

The supernatant was then discarded, and 360 μl of RIPA buffer was added to the remaining nuclear pellet.

The supernatant was then discarded and the cells resuspended in 1 mL of PMN buffer, and total cell number determined using Trypan Blue exclusion on a hemocytometer.

The supernatant was then discarded and the purified LA pellet re-suspended in a buffer solution (1.5 mM CaCl2, 5 mM TRIS) to a final phospholipid concentration of 5 mg/ml.

Media was then discarded, and 50 μl of Promega bright-glo luciferase substrate dissolved in lysis buffer was added to the cells before recording luminescence using a Veritas Microplate Luminometer (Promega).

The water was then discarded.

The supernatant was then discarded.

Any read shorter than 45 bp was then discarded.

The supernatant was then discarded and hepatocyte pellet gently resuspended.