Sentence examples for buffer was then diluted from inspiring English sources

The denaturation buffer was then diluted with an equal volume of 50 mM Tris-HCl, pH 8.0 with 1 mM DTT.

Concentrated proteins (in the same buffers) were then diluted into buffered denaturant-free or denaturant stocks (see Table S2).

The sample was then diluted with Buffer A (5 mM KH2PO4, 10% acetonitrile, pH 3.0) to 20% acetonitrile and fractionated using strong cation exchange chromatography (2.1-mm × 20-cm polysulfethyl A column, Poly LC, Columbia, MD) on an UltiMate 3000 HPLC instrument (Dionex UK Ltd., Surrey, UK).

100 μl of each sample was then diluted with 900 μl ChIP dilution buffer (EMD Millipore).

The sample was then diluted in RLT buffer and ethanol.

Dephosphorylated eEF2K was then diluted in autophosphorylation buffer.

Streptavidin phycoerythrin (25 ×, SAPE, catalog No. 45-001D) was then diluted in Assay Buffer and aliquoted into wells (25 μl).

Each homogenate was then diluted in homogenization buffer so as to equilibrate the protein content in all the samples.

The resulting supernatant was then diluted in PBS buffer for use in fluorescence analysis in clear bottom microtiter plates using a Safire fluorimeter (Tecan, Männedorf, Germany).

The purified protein was then diluted to final buffer conditions of 10 mM sodium phosphate, 110 mM NaCl, 20% glycerol and stored at −80 °C.

This solution was then diluted in HEPES buffer to achieve the desired aptamer concentration (1 μM, the optimal concentration for TGF-β1 binding).