Sentence examples for buffer was then changed from inspiring English sources

The perfusion buffer was then changed to IB with an EGTA-buffered [Ca2+] of 4 μM or 1 μM, eliciting a gradual rise in [Ca2+]m that reached a plateau value of ∼80 and ∼20, respectively.

The perfusion buffer was then changed to IB, which had an EGTA-buffered [Ca2+] of 1 μM.

The eluted protein was concentrated using a VivaSpin 20 concentrator (cutoff MW 10 kDa, VivaScience) and the buffer was then changed to 10 mM phosphate buffer, pH 7.5, NaCl 150 mM (PBS) supplemented with 10% glycerol and 1% OG using a PD-10 column resulting in a final concentration of 10 15 mg/ml as determined by Bearden [46].

The staining buffer was then changed to 70% ethanol for clearing chlorophyll.

The developing buffer was then changed, and the gels were incubated at 37°C for 24 h.

The reaction buffer was then changed to a fresh one and the gels were incubated (24 h, 37°C) in a shaking incubator.

The culture medium was then changed every 2 days and at day 5 post-infection, cells were harvested in lysis buffer.

After binding, the affinity resin was washed with two changes of buffer (20 m M HEPES pH 7.4; 100 m M KCl) and SDS PAGE sample buffer was then added directly to the washed resin.

A two-goal buffer was then established after Catriona Wagg converted another chance.

Solubilisation buffer was then added.

Serial dilutions of rHA (15 μl) in dilution buffer were then added to quadruplicate wells and the change in wavelength recorded 15 times at 2 min intervals.