Sentence examples for buffer was then carried from inspiring English sources

The recovery of V. cholerae J3324 and V. cholerae UVC1324 DNAs at 1.0 × 10−4 μg μL−1, 1.0 × 10−5 μg μL−1, and 1.0 × 10−6 μg μL−1 spiked into the hybridisation buffer was then carried out using the proposed PSA-AuNPs-based electrochemical DNA biosensor.

Second strand cDNA synthesis was then carried out: Klenow buffer plus 0.3  μl of 10  μ M TAGrandom primer (GACTCGAGTCGACATCGAIIINNNNNN where I is Inosine) were added, heated to 70°C 5 min, cooled to room temperature and 40 U Klenow added (25°C 10 min, 30°C 10 min 37°C 1 h, 75°C 10 min).

Prehybridization was then carried out for 3 h in hybridization buffer in a humidified chamber at 42 °C.

The incorporation of either cold Ga3+ or Ga3+ into the NOTA core of complex of Gd6H3 L was then carried out at pH 3 5 in an HEPES buffer.

Size exclusion chromatography was then carried out using a Superdex 200 column in a buffer containing 40 mM HEPES pH 8.0, 150 mM NaCl, 1 mM DTT.

Automatic magnetic separation was then carried out and nucleic acid was recovered in 20 µl elution buffer.

Color development was then carried out using either 0.6 mg/ml DAB or 0.4mg/ml AEC in a buffer solution containing 0.1M sodium acetate pH 4.7 and 0.02% H2O2.

A response surface study was then carried out considering all the critical process parameters, including both the PVs and the mixture components (MCs) of the microemulsion (borate buffer, n-heptane as oil, sodium dodecyl sulphate/n-butanol as surfactant/cosurfactant).

Collagen immobilization was then carried out.

Staining was then carried on as above.

The final bleed was then carried out.