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The efficiency of iron removal from Lf in citrate buffer was tested in a pH range of 2 5 (Fig. 4).
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Antibody samples serially diluted in running buffer were tested in duplicate with the final data sets collated from two separate experiments.
These buffers were tested in different molarities for their capacity to elute rbST from the monolith micro-columns.
The effect of the dilution in the Panbio buffer was tested prior to the use on patient samples and did not show any influence on the results (results not shown).
In vitro reactivity of the BG nanoparticles was tested in phosphate buffer saline (PBS), Tris-buffer (TRIS), simulated body fluid (SBF), and Dulbecco's modified Eagle's medium (DMEM), in comparison with similar sized hydroxyapatite (HA) and silicon substituted HA (SiHA) nanoparticles.
The yPDE1 activity was tested in a buffer of 50 mM Tris-HCl (pH 7.5) and also this plain buffer with 10 mM MgCl2, 2 mM MnCl2, 0.01 mM ZnCl2, 1 mM EDTA, or 1 mM DTT.
The pH optimum of the purified enzyme was tested in HEPES buffer.
The effect of pre-amplification was tested in both buffer and plasma to explore if a less time-consuming protocol could be applied.
Cleavage of fluorigenic substrates of different lengths by full-length or catalytic domain MALT1 was tested in assay buffer plus 1 mM EDTA.
In a mechanistic study, Dox released from the GO vehicle was tested in pH 5.0 buffer (modeling tumor environment) and pH 7.4 buffer (modeling normal physiological environment), respectively, as shown in Figure 3 c.
Fibril dissociation was tested in two buffers, PBS and ABS, both at pH 5.5.
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