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Further, sodium acetate buffer was taken as a variable to optimize the polysaccharide additives.
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Lysis Buffer and reaction Buffer were taken as the blank control.
The solvent viscosity and the refractive index of the buffer were taken as 0.894 cP and 1.33, respectively, at 25 °C.
Untransfected HEK293 cells and wash buffer were taken as the controls, since they showed little to no activity.
Only sample incubated in buffer and not exposed to any oxidant was taken as control for each set.
Triton X-100 (0.1% v/v) was taken as positive control and phosphate buffer saline (PBS) was taken as negative control and pass through the same process.
For each assay, 0.1% Triton X-100 was taken as a positive control, 100% blood lysis and Phosphate buffer saline (PBS) was taken for each assay as a negative control (0% lysis).
Triton-X (0.1%) was taken as a positive control for 100% lyses and PBS buffer as negative control for 0% lyses.
For these results, a cluster of 7 cells is taken, i.e., K = 7 and the buffer size is taken as N = 5.
Here, haemoglobin concentrations of ≥50, ≥75, ≥100, and ≥200 ng/ml of buffer solution were taken as cut-off values.
Five aliquots of 100 μL of the 10 MBq·mL−1 solution of [11C]prucalopride in 0.2 M phosphate buffer (pH = 7.4) were taken as reference for determining recovery.
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