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As described in Section 4.3, the BoNTAe inhibition assay buffer was supplemented by 25 µM ZnCl2, whereas the BoNTBe inhibition assay buffer contained no exogenous zinc ion.
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To enhance sensitivity, reaction buffers were supplemented by the addition of sodium 3,5-dichloro-2-hydroxy-benzenesulfonate.
When optical tweezers were used, the ATP buffer was supplemented with NeutrAvidin-functionalized silica microspheres.
Wild-type and mutant FICD/HYPE were expressed and purified as EfFIC, except that the lysis buffer was supplemented with 1 mM dithiothreitol (DTT) and 0.02 % Triton X-100 and the other buffers were supplemented with 1 mM DTT.
AP1 lysis buffer was supplemented with 2.5%PVP400.
Buffer was supplemented with 100 μM FAD where indicated.
General actin buffer was supplemented with 0.2 mM final ATP.
The buffer was supplemented with 1 mM calcium chloride.
Labeling kit assay buffer was supplemented with 2% FBS.
Lysis and wash buffer were supplemented with 5 mM imidazole and elution buffer supplemented with 300 mM imidazole.
The work was supplemented by local volunteers.
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