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Three scans of the CD spectra of each peptide were averaged and the CD signal from PBSF buffer was subtracted out.
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Bowel fat was subtracted out from the VAT, and L4/L5 measures used in these analyses.
Stimulus artifact was subtracted out by averaging with stimulation at an inverted phase.
The resulting scattering curves for each sample were radially averaged, and the buffer was subtracted.
The spectrum for the appropriate buffer was subtracted.
The background fluorescence spectrum of the buffer was subtracted.
The contribution of buffer was subtracted from experimental spectra.
The spectrum for the buffer was subtracted from the spectrum of the protein.
ThT background signal from buffer was subtracted from corresponding measurements.
The scattering of the buffer was subtracted, and the difference data were extrapolated to zero solute concentration following standard procedures.
All measurements were performed in triplicate, and the fluorescence intensity of the buffer was subtracted from that of the samples.
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