Sentence examples for buffer was substituted with from inspiring English sources
Whole cell extracts were prepared as described previously except that the standard lysis buffer was substituted with HB buffer (25mM Tris-HCl [pH 7.5], 15 mM EGTA, 15 mM MgCl2, 0.1% NP40, 1 mM DTT, 0.1 mM NaF).
However, if the Prochlorococcus cultures also contained EZ55, or if the HEPES buffer was substituted with the buffer TAPS, which generates much less HOOH (Figure 1C), HOOH concentrations were maintained at or below 0.1 µM, and subsequently Prochlorococcus cells survived, doubling about once before running out of nutrients in this unamended seawater medium (Figure 1D).
Furthermore, there was no significant effect on ApST4 transport activity when NaCl in the transport buffer was substituted with choline chloride.
For control reactions buffer was substituted for the enzyme.
In this case the guanidinium thiocyanate buffer was substituted by a buffer containing 0.6 M NaCl, 10 mM EDTA, 4% SDS, 100 mM Tris, pH8) and after phenol extraction a precipitation step with 8 M LiCl was introduced.
Unmodified Kar2 control proteins were prepared identically except buffer was substituted for NEM or CHP.
The arteries were equilibrated for 30 min before being stimulated three times with a depolarising stimulus of 87 mM K+-HEPES-Tyrode buffer (K+HT), in which NaCl was substituted with KCl to maintain an iso-osmolar solution.
Cells were maintained in continuous perfusion with the chloride-containing buffer (solution #1): after 340 sec, solution 1 was substituted with a Cl− free buffer (solution # 2) containing NaNO3 140 mM, KNO3 5 mM, gluconic acid 3 mM, MgSO47H2O 1 mM, glucose 5 mM, HEPES 20 mM, pH 7.4.
After 50 min of electrophoresis, 'dark cathode buffer' (Invitrogen) containing 0.02% Coomassie Brilliant Blue G-250 was substituted with 'light cathode buffer' (Invitrogen) containing 0.002% Coomassie Brilliant Blue G-250.
For SDS-PAGE analysis, the supernatant was substituted with Laemmli buffer (Laemmli 1970).
In addition, control experiments were performed where the primary antibody was substituted with blocking buffer to establish the specificity of the secondary antibodies.
