Sentence examples for buffer was substituted for from inspiring English sources

The activity measured in the special unit (WAU) was calculated with the equation (t c  − t)/t, where t is the time required for the drop in two units of pH from 8.3 to 6.3 was measured, while t c (control) is the time required for the pH change when buffer was substituted for the test sample.

For control reactions buffer was substituted for the enzyme.

Unmodified Kar2 control proteins were prepared identically except buffer was substituted for NEM or CHP.

The procedure for the hepatic microsomal enzyme assay (for ATRA 4-hydroxylases) was used where 100  μl of cellular CYP26 microsomes (500  μg ml−1 dissolved in storage buffer) was substituted for 100  μl of hepatic microsomes.

A final concentration of 2% paraformaldehyde was used and phosphate buffered saline was substituted for sperm salts.

Beads were washed two times in Triton Wash buffer, then washed an additional two times in glycerol wash buffer (same composition as Triton wash buffer except 20% glycerol was substituted for 0.1% Triton X-100).

The Calmodulin resin was washed 30 ml of binding buffer and the bound proteins eluted with 5×200 µl fractions of calmodulin elution buffer (2 mM CaCl2 was substituted for 2 mM EGTA).

Because of the high sugar and phenolic content of the tissues, Qiagen RLC buffer (2% polyethylene glycol (MW 20,000), 0.2 M sodium acetate (pH 5.2), and 1% β-mercaptoethanol was substituted for lysis buffer.

Note: (1) TRIzol® Reagent was substituted for lysis buffer provided in extraction kit.

HRP signal was developed with a DAB substrate kit (Vector Labs, Burlingame, CA) according to the manufacturer's instructions, except that PBST was substituted for the buffer provided.

As a negative control, cell extract was substituted for lysis buffer.