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In this case the guanidinium thiocyanate buffer was substituted by a buffer containing 0.6 M NaCl, 10 mM EDTA, 4% SDS, 100 mM Tris, pH8) and after phenol extraction a precipitation step with 8 M LiCl was introduced.
Similar(59)
However, in order to eliminate interference of amines during labelling, buffers were substituted by phosphate buffers (wash buffer: 5 mM KPO4 pH 8.0,80%% ethanol; elution buffer: 4 mM KPO4 pH 8.5).
For control reactions buffer was substituted for the enzyme.
Unmodified Kar2 control proteins were prepared identically except buffer was substituted for NEM or CHP.
"It was substituted by a lottery mentality.
For example, 'globalisation' was substituted by 'international'international
The nettle linguine was substituted by regular pasta.
Mycophenolate mofetil was substituted by azathioprine.
In the Cl−-free media, chloride was substituted by gluconate.
Here, the disinfectant was substituted by water of standardized hardness.
Wine consumption (component 7) was substituted by any alcohol consumed.
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