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The blocking buffer was subsequently removed and the plate was washed with PBS-T three times.
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TCEP was subsequently removed by buffer exchange using a PD-10 desalting column (GE Healthcare).
Imidazole was subsequently removed by extensive washing in annexin V binding buffer (1.8 m M CaCl2, 10 m M HEPES-NaOH pH 7.4, 150 m M NaCl, 5 m M KCl and 1 m M MgCl2).
The digitonin was subsequently removed by washing for 3 × 3 min with transport buffer.
Unspecific bound probe was subsequently removed by incubating at 48°C for 15 min in appropriate washing buffer.
The "asterisk" was subsequently removed.
The oxygen demand was subsequently removed in lower reactor sections.
500 μL of binding buffer (PBS containing 1% BSA and 0.1% sodium azide) was added to cell on ice for 10 min and the supernatant was subsequently removed from cells.
The cellular debris was subsequently removed by washing with PBS.
The MT tube was subsequently removed and tracheal intubation performed.
A third contestant was subsequently removed by ITV.
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