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After lysis, some lysate was kept for the input fraction and after the addition of 2× SDS buffer was stored in −20°C.
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Video buffers are stored in a DDR-SDRAM, and memory access is implemented with the multi-port memory controller (MPMC) Xilinx fast memory controller.
For cells subjected to hypoxia, PBS and KRH buffers were stored in an atmosphere of 1%O2/94%N2/5%5% CO2 prior to addition to the cells.
The HSA stock solution was dissolved and diluted to 1.0 × 10−5 mol L−1 with the same buffer, then was stored in the dark at 4 °C before fluorescence and UV vis absorption essay.
The interface was then covered with 5 μL of 1 mM MCH (in PBS) and kept at room temperature for 30 min. After rinsing with PBS buffer, the sensing platform was stored in PBS buffer for at least 20 min prior to experimental measurements.
We prepared a 6 × stock of 1.5 mM 12-pNCA in 36% dimethyl sulfoxide (DMSO) and the EPPS buffer (the 12-pNCA was stored in the DMSO solution and combined with the buffer immediately before use).
The purified and concentrated protein was stored in buffer containing 50 mM Tris HCl, pH 7.4, and 10% glycerol at −20°C.
The refolded protein was stored in buffer containing 0.05 M Tris (pH 8.0), 0.15 M NaCl, 0.5 M Urea and 5% glycerol.
When WIV was stored in buffer solution, the IgG2a-dominated antibody response was preserved at all storage temperatures and storage durations tested (Table II).
Aliquots of SimReg1 fusion protein in storage buffer were stored at - 80°C, or used immediately in DNA-binding assays.
Genomic DNA was isolated from peripheral blood lymphocytes using standard phenol-chloroform method and then was stored in TE buffer.
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