Sentence examples for buffer was stored at from inspiring English sources

As 'input', 40 μl lysate +25 μl 4× loading buffer was stored at −20°C.

After removal of cell debris by centrifugation at 250 ×g for 10 min, the supernatant was centrifugated at 20,000 ×g and 4°C for 20 min and the pellet, resuspended in SPG buffer, was stored at −70°C.

Aliquots of SimReg1 fusion protein in storage buffer were stored at - 80°C, or used immediately in DNA-binding assays.

Samples in SM buffer were stored at 4°C until further use.

MACS-sorted prostate cell lysates in RLT buffer were stored at -80C for no more than a month.

Final protein preparations in Laemmli buffer were stored at -20°C until use. 3 μg of DNase-digested total RNA was used for oligo (dT) primed first strand cDNA synthesis with SuperScript amplification (Invitrogen) following manufacturer's suggestions.

Buffers were stored at 4°C until use.

All stock buffers were stored at 4°C and were kept for 1 month.

The buffer solution was stored at 4 °C before use.

A standard curve was generated by preparing a stock of 10 mM NaHPO4 in assay buffer which was stored at -20°C when not in use.

Final elution was performed with 30 μL M-Elution Buffer (Zymo Research) and was stored at −70°C until use.