For evaluation of slug migration and phototaxis 5×10 cells in 5 μl Soerensen phosphate buffer were spotted in the center of a water agar plate.
Purified PCR products, in 3 × SSC buffer, were spotted in duplicate on MicroMax SuperChip™ I slides (Perkin-Elmer Life Science, Boston, MA, USA) at the Leiden Genome Technology Center (http://www.lgtc.nl/) using the OmniGrid 100 robot (GeneMachines, San Carlos, CA, USA).
Serial dilutions of plaques resuspended in phage buffer were spotted in 10 μl aliquots on the tester strains plated on BBL top agar supplemented with carbenicillin, 100 μg/ml.
L1 larvae synchronized by sodium hypochlorite treatment and overnight starvation in M9 buffer were spotted on plates and grown until day 1 of adulthood.
The seed extracts with and without buffer were spotted as controls.
Each oligonucleotide was spotted in quadruplicate, while α- and β-tubulin were spotted at the corners of the grid and reference oligonucleotides and additional spotting controls (empty, water, spotting buffer) were distributed randomly.
DNA was resuspended to 150 ng/μl in spot buffer (150 mM sodium phosphate, 0.01% sodium dodecyl sulphate) before being spotted in duplicate on to amino-silane coated GAPSII slides (Corning) using a Biorobotics MicroGrid II spotter (Genomic Solutions).
The cDNA were spotted in tandem on GapsII amino-silane slides (Corning) in 3× SSC and 1.5 M Betaine buffer using Stealth Micro-Spotting Pins (Telechem) at 20°C and 65% humidity.
Genes are spotted in quadruplicate.
BACs were spotted in quadruplicate.
