Sentence examples for buffer was sonicated for from inspiring English sources

For control experiments in the absence of H2O2 and oxygen, 100 mL of the buffer was sonicated for 60 min, and residual oxygen was removed by purging with nitrogen.

The blood mixed with buffer was sonicated for 3 min, and its fluorescence measured with a fluorescence microplate reader (Tecan Japan Co., Ltd).

One hundred microliters of brain homogenate in HS buffer were sonicated for 5 min using a water-bath sonicator and then centrifuged at 100,000 × g for 30 min at 4 °C.

The resuspended cells in 500 μL of the same buffer were sonicated for five pulses of 30 s and pauses of 30 s on ice with a sonicator (Laballiance SL-600S, USA).

Cell paste in start buffer was sonicated on ice for 20 min. After centrifugation (23700 g for 30 min, Beckman JA-20 rotor), the supernatant was filtered through a 0.45 μm syringe filter.

Solution of 1.5 mg FMNPs-COOH was dispersed in 2 mL pH 7 PBS buffer and was sonicated for 10 min. Then we mixed 1 mL of fresh 400 mM EDC and 100 mM NHSS in pH 6.0 MES buffer and rotated it at room temperature for 15 min. After this, the resulting solution was separated by magnetic field, and 1 mg/mL of HAI-178 monoclonal antibody was added to the above mixture and stirred in dark place for 2 h.

To visualize DNA origami block polymerization on small unilamellar vesicles, a 1 mg/mL DOPC solution in 10 mM HEPES buffer (150 mM NaCl) was sonicated for 15 min with a tip sonicator.

The cells or spinal cord lysates of the transgenic mice in TNE lysis buffer were sonicated and centrifuged at 15 000 × g for 5 min at 4 °C.

The content of the flask was sonicated for 20 min then diluted to volume with bicarbonate buffer.

The corresponding buffer mixture and unlabelled sugar donor were added, the mixture was shaken vigorously, and each sample was sonicated for 30 s.

The mixture was sonicated for 2 min.