Sentence examples for buffer was sheared into from inspiring English sources

In brief, 100−400 ng of genomic DNA in 50 µL of volume of low TE buffer was sheared into fragments of approximately 300 base pairs with the Covaris S2 or E210 system (Covaris, Inc. Woburn, MA).

The mRNA was sheared into short fragments with fragmentation buffer and selected for construction of Illumina RNA-Seq libraries according to the described protocol.

Cells were then collected by centrifugation and lysed in SDS lysis buffer (1% SDS, 10 mM EDTA, 50 mM pH 8.0 Tris-HCl, and protease inhibitors); after sonication, DNA was sheared into 0.3 2 kb fragments; insoluble materials were removed by centrifugation.

The circular DNA was sheared into DNA fragments ranging from 300 to 800 bp by nebulization.

For every sample, 200 ng was sheared into ~250 bp using S220 Focused ultrasonicator (Covaris, MA).

Mitochondrial DNA was sheared into blunt ended fragments by enzymatic lysis using Ion Shear Plus Reagents Life Technologiess).

Briefly, 2 μg of each genomic DNA was sheared into 100-500 bp fragments.

UM 226 genomic DNA was sheared into smaller fragments by Covaris S/E210 or Bioruptor.

An entire genome is sheared into short fragments, which are then amplified and sequenced.

In a first step, genomic DNA is sheared into small random fragments.

The purified products were sheared into random fragments of approximately 1.5 kb and cloned into the pMD18-T vector (TAKARA).