Sentence examples for buffer was separated from from inspiring English sources
In order to avoid a reaction between the water fugacity buffer and the specimen, a double-capsule assemblage was designed such that the water fugacity buffer was separated from the specimen by an inner metal jacket permeable to hydrogen and impermeable to other components.
The labelled probe, dissolved in 50 µl of TE buffer, was separated from unincorporated nucleotides by passing through an Illustra Nick column (GE Healthcare, Buckinghamshire, UK) and heat denatured as described by the manufacturer.
The small pieces of seminiferous tubules were collected, placed in fresh PEM/250 buffer and epithelium was separated from tubule walls as described elsewhere (Guttman et al., 2007).
The top coating was separated from the buffer layer by a distinct epitaxially grown austenitic layer.
The transition zone, which was separated from the buffer layer by Type II boundaries, was comprised of a narrow austenitic martensitic band.
Cells were lysed with lysis buffer and fragmented double stranded DNA was separated from chromosome-length, unfragmented DNA followed by trichloroacetic acid (TCA) precipitation (Duke and Cohen, 1992).
The DNA protein complex was separated from beads by extraction buffer, then DNA and protein were reverse cross-link with Proteinase K and NaCl overnight at 65 °C.
After 12 h, labelled protein was separated from free fluorophore by dialysis technique (Additional file 1), labelled protein contain fluorophore was dialyzed in phosphate buffer (pH: 7) to separate out unconjugated fluorophore from conjugated one.
The beads were washed three times with lysis buffer and immunoprecipitates were separated from the beads by adding 2× sample buffer and boiled.
