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A reference sample of the same serum dilution with buffer was run in tandem with each patient sample to correct for bulk RI changes inherent to the sample.
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Full-length, in vitro transcribed segment 7 RNA, serially diluted in buffer, was run with each assay to give a semiquantitative estimate of the viral load in each tissue.
A sample of the peptide dissolved directly in the loading buffer was run for comparison with material that had been extracted from the air water interface.
A gradient of 95% buffer A (100 mM triethyl-ammonium-acetate aqueous solution) and 5% buffer B (25% acetonitrile in 100 mM triethyl-ammonium-acetate aqueous solution) to 100% buffer B was run in 20 min.
The SPR assay was run in the running buffer (20 mmol/L Tris-HCl, pH 7.5, 200 mmol/L NaCl and 0.005% Tween 20) at the flow rate of 30 μL/min.
The column was run in buffer containing 20 mM Tris/HCl (pH 7.8), 150 mM NaCl, 5% (v/v) glycerol and 1 mM 2-mercaptoethanol while collecting 2.5 ml fractions.
The gel was run in 1× MOPS buffer at 5 V/cm with regular buffer mixing.
Affinity purified CCN3/NOV was fractioned with a bipartite chromatogram when the gel filtration was run in a buffer that simply contained 10 mM Tris (Figure 3A).
The gel was run in TAE buffer initially for 1 h at 40 V followed by 2 hours run at 60 V.
In the neutral version, electrophoresis was run in a buffer consisting of 100 mM Tris and 300 mM sodium acetate at pH adjusted to 9.0 by glacial acetic acid (Singh and Stephens 1997).
Gel (acrylamide mass fraction 12%) was run in 0.01 KMgTB buffer and then UV-shadowed.
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