Sentence examples for buffer was resolved by from inspiring English sources

The beads were washed with Co-IP buffer, and captured proteins eluted from the beads using loading buffer was resolved by SDS PAGE for western blot analysis using a monoclonal TDP-43 antibody (Proteintech, 60019-2-Ig).

For WB of IGF-I (Fig. 5), 1.0 μL plasma samples diluted in reducing Laemmli buffer was resolved by PAGE (4 20% Criterion TGX Bio-Radd) and transferred to PVDF membranes.

20 μg to 30 μg of total protein in SDS sample buffer was resolved by SDS-PAGE (10% or 12.5%) and transferred to PVDF membrane using a semi-dry transfer system (Hoepher SemiPhor).

Brain, liver, and spleen homogenates (10%) prepared in lysis buffer were resolved by SDS-PGE and subjected to western blotting using specific antibodies as described in previous reports [30].

Proteins in Laemmli sample buffer were resolved by SDS-PAGE (10%) and transferred to PVDF Immobilon-P membrane.

Cell lysates extracted in RIPA buffer were resolved by 3 8% SDS-PAGE.

Cells lysates, prepared by dissolving washed cells directly in SDS sample buffer, were resolved by SDS-PAGE with indicated percentage of acrylamide.

Whole-cell protein extracts prepared in Laemmli sample buffer were resolved by SDS-PAGE using NuPage 4 12 % gels (Invitrogen), transferred to Invitrolon polyvinylidene difluoride membranes (Invitrogen), and probed with primary and horseradish peroxidase-conjugated secondary antibodies.

Whole-cell protein extracts prepared in Laemmli sample buffer were resolved by SDS-PAGE using 4% to 12% polyacrylamide gels (NuPage; Invitrogen Corp ., transferred to PVDF membranes (Invitrolon; Invitrogen Corp ., and probed with primary and horseradish peroxidase-conjugated secondary antibodies.

2 μl of 2× protein sample buffer was added to the input and unbound aliquots, which were then heated at 95°C for 5 min. These samples along with a bound sample (10 μl of the bound fraction plus 10 μl of 2× protein sample buffer) were resolved by SDS-PAGE on a 7.5% polyacrylamide gel.

Total cell protein, extracted using RIPA buffer, were resolved by SDS-PAGE (8%) in parallel duplicated gels, transferred to nitrocellulose membranes and incubated with primary antibody ((Phospho-EGFR Tyr-1045, or total EGFR; Cell Signalling Technology) diluted 1/1000 in 5% BSA in TBS-T overnight at 4°C.