Sentence examples for buffer was replaced with from inspiring English sources
Buffer was replaced with fresh HEPES-PBS and cells were further incubated at 37 °C for 40 min prior to a second extensive rinsing.
For buffer desalting, the ribosome dilution buffer was replaced with PBS through ultrafiltration (Amicon Ultra 3000 Da MW; Merck, Darmstadt, Germany).
After 3 h, the buffer was replaced with fresh KRB with no glucose, 2.5 mM (cells/PIs) or 2.8 mM (murine islets) (basal level) and 25 mM (cells/PIs) or 20.2 mM (murine islets) glucose (supraphysiological level) as stated and cells/islets were further incubated with or without illumination.
For stage 2, further optimisations were applied to methods D and N as follows: The single AW buffer wash step in method D was increased to three washes, and the supplied elution buffer was replaced with 2 × 50 μL 10 mM Tris (pH 8.5).
After each time point, the plates were placed on ice, and the ligand binding buffer was replaced with ice-cold strip/stop solution.
For testing the effect of Mn++, Mg OAC 2 (5 mM) in the buffer was replaced with varying concentrations of MnCl2 (0.5, 1, 5, 7.5 mM).
After that, the buffer was replaced to TE buffer with no supplement and stored at 4 °C.
Then, binding buffer was replaced and cells were stained with PI.
Then, the buffer was replaced by fresh KRBB solution supplemented with 5 mM glucose or 25 mM glucose and cells were incubated at 37 °C for another hour.
The samples were washed five times with MOPS buffer, which was replaced with fresh AP buffer for 30 minutes before determining the location of AP-tagged anti-Dig antibody location was detected by incubation in nitroblue tetrazolium/5-bromo-4-chloro-3-indolyl phosphate dissolved in AP buffer (pH 9.0) containing 10% dimethyl formamide overnight at AT.
