Sentence examples for buffer was replaced by from inspiring English sources
To covalently conjugate 17G1 (or mouse IgGs) to 1,4,7,10-tetraazacyclododecane-1,4,7,10-tetraacetic acid (DOTA), the mAb buffer was replaced by 0.1 M sodium carbonate, pH 9.0, by overnight dialysis (Spectrum Laboratories; CA, USA) or centrifugal ultrafiltration (Millipore) at 4 °C.
After laser illumination of CaM-A488 (and re-incubation with CaM-A647) the buffer was replaced by PBS (without ascorbic acid).
After 1.5 hours, the cathode buffer was replaced by anode buffer, and electrophoresis continued for a further 5 hours at 8 mA (120 V).
The HEPES buffer was replaced by MES in acidic solutions (pH 5.0 6.5) with AMPSO (8.5 9.0) and by CAPS in alkali solutions (pH 9.5 11.0).
To measure the intracellular calcium concentration, rMC-1 cells were incubated with 5 µM Fluo-4/AM (Invitrogen, Carlsbad, CA) at 37°C for 30 min. Then the incubation buffer was replaced by DMEM containing 1% FBS.
Transpososome assembly mixtures and quantification of the complexes by EMSA was as previously described [29], [34] except that the Tris buffer was replaced by Hepes pH 7.5 and DTT was omitted from all solutions.
We found that the carboxyl methylation reached a plateau with its maximum yield within 3 h incubation at 30 °C when Zn2+/Mn2+-containing buffer (buffer P) was replaced by 2.5 mM LiCl/5 mM KCl-containing buffer C. The final product of POG-modified, VIS-cleaved, and carboxyl methylated Tβγ (designated POG-Tβγ-OMe) was purified by MonoQ column chromatography.
Additionally, the original 70 mM buffer system was replaced by a 10 mM buffer system when B. subtilis was cultivated in a benchtop bioreactor allowing pH control.
To access 20S function, buffer I was replaced by an ATP-free buffer containing SDS (20 mM HEPES, pH 7.8; 0.5 mM EDTA, 0.03% SDS) [ 10].
For the ATPase activity of reconstituted BmrC/BmrD (3 µg), buffer B was replaced by 50 mM Hepes pH 8 and measurements were done at 37°C.
l-[3,4-H] glutamate (50.6 Ci/mmol, PerkinElmer Life Science, Boston, MA, USA) was added to a final concentration of 0.05 μM for an additional 20 min, following which the assay was terminated with two washes in ice-cold Na+-free assay buffer (NaCl was replaced by equimolar LiCl).
