Sentence examples for buffer was removed with from inspiring English sources

All but three wells were masked with tape, the three open wells were pre-rinsed by soaking for 1 min with pH 4.9 Britton-Robinson universal buffer [ 1] and the buffer was removed with vacuum suction.

Fixation buffer was removed with two washes with permeabilization buffer (BD Pharmingen) and samples were split and subsequently stained for intracellular cytokines using 1/200 anti-IFN-γ-allophycocyanin, anti-IL-4-PE, anti-IL-10-allophycocyanin, anti-IL-13-allophycocyanin, anti-IL-17-PE, or the relevant isotype control for 20 min in Perm buffer.

Staining buffer was removed and replaced with staining buffer supplemented with 5-bromo-4-chloro-3-indolyl-beta-D-glucuronic acid (X-Gluc) to a final concentration of 2 mM.

The buffer was removed and replaced with HBSS buffer (control) or HBSS with IgM or FITC-labeled human IgM (10 µg/ml) and the cells were incubated for 24 h at 37°C with 5% CO2.

Once the gel plugs had become swollen with absorbed digestion buffer, the excess buffer was removed and replaced with the same buffer without trypsin.

Tris-NaCl-blocking buffer was removed and replaced with anti-human RANK N-2B10.1 N-2B10.18.1; Amgen, Seattle, WA) or RANKL (M366; Amgen, Seattle, WAmgenuSeattleclonal antibodies or isotype-matched control mouse IgG (BD Pharmingen, San Jose, CA) at concentrations of 5 μg/mL for anti-RANK and 1 μg/mL for anti-RANKL for 60 minutes.

The last of the 5 PBT washes was aspirated and replaced with equilibration buffer for 20 minutes at RT. Equilibration buffer was removed and replaced with working enzyme solution (50-100 μL of liquid per 20 embryos).

After 20 min at 37°C, the K2SO4 buffer was removed and replaced with DMEM and the parasites were incubated for 5 min more at 37°C.

The fixative solution was removed, the pellet was washed in 0.1 M HEPES buffer (pH 7.3) twice for 5 minutes each, and the buffer was removed and replaced with a solution containing 1% osmium/1.5% potassium ferricyanide and incubated at room temperature in the dark for 30 minutes.

Blocking buffer was removed and replaced with the test proteins and incubated at 37°C for 1 h.

The buffer was removed and replaced with 2 μM miRNA-specific LNA probe or scrambled probe (Exiqon, Denmark) labelled with digoxygenin (DIG) in hybridisation buffer and incubated in a humidifying chamber at 50°C for 18 hrs.