Sentence examples for buffer was removed by from inspiring English sources
After the buffer was removed by filtration, the resin was shaken in a HEPES-buffered solution containing Zn(NO3 2 ⋅6 H2O (0.073 g, 0.24 mmol) at room temperature for 24 h.
After 10 min on ice, the buffer was removed by aspiration.
Blocking buffer was removed by capillary action and the slides incubated over night at 4°C in either IP-10 goat polyclonal IgG (Santa Cruz G-15 sc-14641) or control goat IgG (Santa Cruz sc-2028) in 2% BSA.
Imidazole of the elution buffer was removed by dialysis.
Excess stop buffer was removed by washing the slide once in 50 mL of PBS for 2 minutes.
At the end, the residual Offgel IEF buffer was removed by pipetting, and the electrode pads were removed.
Staining with anti-F4/80 antibody (diluted 1 : 400) conjugated with PE-Cy7 rat anti-mouse antibody was done for 30 minutes; antibody and staining buffer were removed by one time of washing in Hank's complete for ten minutes at 4°C and 500 g without brake and suspending in the same solution.
Contaminating enzymes and buffers were removed by purification with the Nucleospin PCR Clean-up Kit (Macherey-Nagel, Ger).
Immediately buffer was removed followed by addition of cold 30% ethanol on ice for 15 min.
This improved the signal-to-noise ratio because noise originating from additional crystal layers or surrounding buffer was removed in silico by careful selection of the z-slices and Fourier filtering of the diffraction pattern.
