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Buffer was removed as completely as possible with a micropipette tip, and then 30 µl Hoyer's medium (30 g gum arabic, 50 ml H2O, 200 g chloral hydrate, 20 g glycerol) was added.
To maintain the highest protein concentration possible in the initial cell lysate, the remaining buffer was removed as much as possible by tilting the plate and using a pipetman.
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The wash buffer was removed from the filter as above, the glass syringe filter with the bound DNA attached to a new 5 ml syringe and 1 ml of TE (pH 8.0) was added.
Once all dissections were done, buffer was removed and fixative was added as described below.
Then buffer was removed and oocytes were fixed by addition of fixation buffer as described above.
Then, the Tris HCl buffer was removed.
Next, the pre-hybridization buffer was removed, and hybridization buffer containing 50 pmol/ml labeled probes was added.
Excess buffer was removed, leaving only interstitial buffer between packed eggs.
Then, the buffer was removed.
Staining buffer was removed and cells were washed with buffer.
The buffer was removed after vortexing.
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