Sentence examples for buffer was read at from inspiring English sources

The absorbance of the samples added to the DMMB buffer was read at 595 nm with a fluorescence reader (Molecular Devices).

Off line alkaline hydrolysis and neutralization of 5% aliquots and ninhydrin colouring in an acetate/cyanide buffer was read at 570 nm [ 8].

Cells were permeabilized using citrate buffer and absorbance was read at 660 nm using a plate reader.

The plates were washed again, and the signals were developed with 100  μl per well of 0.03% o-phenylene diamine dihydrochloride, 0.02% hydrogen peroxide, and 0.15 ℳ citrate buffer, and absorbance was read at 490 nm using an enzyme-linked immunosorbent assay (ELISA) reader (Benchmark Microplate Reader; Bio-Rad, Hercules, CA, USA).

The fecal extracts were diluted 1 50 with incubation buffer and the absorbance was read at 450 nm in a microtiter plate reader.

The dye was extracted with 10% cetylpyridinium chloride monohydrate (Sigma-Aldrich) in 0.1 M phosphate buffer (pH 7.0) and the absorbance was read at 550 nm (Perkin Elmer Victor X3 microplate reader).

Protein-bound precipitates were dissolved in 10 mM Tris buffer (pH 10.5) (Merck), and the plate was read at a wavelength of 492 nm (Multiskan Spectrum Microplate Reader; Thermo Labsystems, Waltham, MA, USA) to determine the cell viability.

In summary, 1 mL Tris-EDTA buffer was added to 50 μL of BALF and sample absorbance was read at 412 nm against Tris-EDTA buffer alone (A1).

Briefly, 1 ml Tris-EDTA buffer (pH = 8.6) was added to 50 μl muscle homogenate in 2 ml cuvettes and sample absorbance was read at 412 nm against Tris-EDTA buffer alone (A1).

After incubation, 70 µl of glycine buffer (0.2 M, pH 10.7) was added and the absorbance was read at 405 nm.

The absorbance was read at 405 nm against a blank containing human plasminogen, Tris buffer, and AAS but without rSK solution.