Sentence examples for buffer was prepared in from inspiring English sources

Assay buffer was prepared in either H2O or D2O.

The calcium-free buffer was prepared in the same manner while CaCl2 was not included.

A total of 50 mM ammonium formate buffer was prepared in water and its pH adjusted to 3.0 with formic acid.

Deuterated cADO assay buffer was prepared in 99.8% D2O with 100 mM D-HEPES (pD 6.8) and 100 mM KCl. Proteated and deuterated buffers were purged with nitrogen for 2 h before being transferred to an anaerobic chamber (Coy Laboratory Products Inc., Grass Lake, MI), where they were allowed to equilibrate for at least 0.5 h before being used.

Sensor solutions in assay buffer were prepared in bulk in buffer containing 50 mM Tris, 10 mM MgCl2, 1 mM EGTA, 2 mM DTT, 0.01% Brij-35P, and 1 mM ATP (final assay concentrations) and aliquoted across 96-well plates.

To determine the LC99.9 values of LL-37 and BP2, 25-μl aliquots of two-fold serially diluted peptide in incubation buffer were prepared in polypropylene microtiter plates (Costar Corning) and to each of the wells, 25 μl of a bacterial suspension containing 2 × 10 CFU/ml was added.

All purification and dialysis buffers were prepared in sterile endotoxin-free water.

The assay buffers were prepared in KCl-HCl buffers (pH 1.1 2.2) and sodium citrate acid buffers (pH 2.2 8.0).

Unless otherwise noted, all samples and buffers were prepared in deionized water prepared using a Milli-Q water purification system (Millipore Corp., Bedford, MA, USA).

All buffers were prepared in deionized water; filtered through 0.2μm membrane, and degassed for 15 min. Column and all buffers were equilibrated to room temperature before use.

CPB buffers were prepared in the range of pH 4.0 7.5 and the reactions were performed at 30, 37, 45, 55 and 65°C.