Sentence examples for buffer was prepared from from inspiring English sources
The acetate buffer was prepared from CH3COOH and CH3COONa (pH ≈ 5.5).
The sample buffer was prepared from 0.5 M Tris HCl buffer pH 6.8, 10%% (w/v) glycerol, 0.5 % (w/v) SDS, bromophenol blue and 5%% β-mercaptoethanol.
Potassium phosphate buffer was prepared from dipotassium phosphate and potassium diphosphate, with the pH adjusted with potassium hydroxide or phosphoric acid.
The acetate buffer was prepared from acetic acid (glacial 99.8% AnalaR grade, BDH, Poole, UK) and sodium acetate (99+% ACS grade, Aldrich, Gillingham, UK).
The ammonium buffer was prepared from ammonium chloride (99.8% AnalaR grade, BDH) and ammonium hydroxide (28 30% NH3 ACS grade, Aldrich).
The cathode buffer was prepared from a 20×stock: 500 mM Tricine, 150 mM Bis-Tris, pH 7.0 and 0.02% (w/v) Serva Blue 27 G-250, supplemented with 0.02% n- D-maltoside.
The assay for bovine milk CRP was carried out according to the manufacturer's instructions; diluent buffer and wash buffer were prepared from the stock of 10 x and 20 x solution respectively, using milli Q water according to the manufacturer's instruction.
All buffers were prepared from highly purified Milli Q water and made RNase-free by treatment with DEPC (diethylpirocarbonate) (Sigma Aldrich).
Buffers were prepared from pH 4.5 to 7.5 in 0.25 pH increments and checked with a portable pH meter (PHM 201, Radiometer Analytical, Brønshøj, Denmark).
