Sentence examples for buffer was prepared for from inspiring English sources

The buffer was prepared for one IPG strip of 7 cm, 50 μL of buffer including 12 mg (8 M) urea, 0.075 mg thiourea, 2 mg 4% CHAPS, 2.5 μL 100% (1 M) DTT, 0.2 μL 1% IPG buffer (ampholines), 20 μL Bromophenol blue, and dionized water up to 125 μL.

Two mixtures of standard proteins containing cytochrome c (0.1 mg/mL), myoglobin (0.1 mg/mL), and beta casein (0.5 mg/mL) dissolved in 0.1% (v/v) FA buffer and 10 mM ammonium acetate (pH ∼6.5) buffer were prepared for conventional and dynamic pH junction based CZE-MS/MS analysis.

Standard buffers were prepared for both running (10X; Tris-Cl (30 g); Glycine (144 g); SDS (10 g)) and transfer buffer (10x; Tris-Cl (30 g); Glycine (144 g)).

DAB working solution made up of 1 20 DAB chromogen in DAB substrate buffer was prepared and applied for 5 min.

For non-reducing conditions, the Laemmli buffer was prepared at pH 6.8 with no β-mercaptoethanol.

The buffer was prepared at room temperature.

A hybridisation buffer master mix was prepared for 3 library captures at room temperature containing 75 uL SureSelect hybridisation buffer #1, 3 uL SureSelect hybridisation buffer #2, 30 uL SureSelect hybridisation buffer #3 and 39 uL SureSelect hybridisation buffer #4.

Total lysates using radioimmunoprecipitation assay (RIPA) buffer were prepared and immunoblotted for protein expression studies using specific primary antibodies and appropriate secondary antibodies.

Samples consisting of serial dilutions of BoNT/A holotoxin in phosphate gelatin buffer were prepared, blinded, and shared with Sandia National Laboratories for parallel analysis via SpinDx, ELISA, and the live mouse bioassay on the same day.

Immunocomplexes were washed four times with NP-40 buffer containing 500 mM NaCl or RIPA buffer, and were prepared for Western blot analysis by boiling in 2× Laemmli loading buffer prior to electrophoresis on an 8% polyacrylamide gel.

Distance from these structural features have relative influence on the landslide, accordingly buffer map is prepared for landslide hazard zonation.