Sentence examples for buffer was prepared as from inspiring English sources

Lysis Buffer was prepared as 15 mM Tris HCl, pH 7.6, 150 mM NaCl, 10% Glycerol, 1% TritonX 100, 1 mM EDTA supplemented with phosphatase inhibitors (5 mM NaF, 0.4 mM Na3VO4, 1 mMNaPPi, 0.1 mM ZnCl2, 1 mM NaMOb) and protease inhibitors (0.2 mM AEBSF, 10 µM Leupeptin, 1 uM Pepstatin A and 0.8 mM Benzmaidine.

Fluorescence activated cell sorter (FACS) buffer was prepared as 1% PBS, 5% FBS, and 0.05% 3 M NaN3.

The reaction buffer was prepared as recommended by the manufacturer.

In short, hybridization buffer was prepared as described by Church and Gilbert (1984), but without BSA.

MTC without MOPS buffer was prepared as described previously [ 46] and contained 5 g/L urea as the nitrogen source.

The hybridization buffer was prepared as described [ 93], and the final washing condition for the membranes was a 20 minute rinse in a 0.2 × SSC and 1% SDS buffer.

Matrix-Lysis buffers (sucrose + protease buffer, lysing buffer 8 M urea + 1% SDS, washing buffer) were prepared as previously described [ 26].

The buffer was prepared at room temperature.

All buffers were prepared as aqueous solutions.

Simultaneously, a reference sample containing 35 ng of DNA in AS buffer was prepared.

Reactions were performed in buffer (15 mL) containing 1 a (50 mg, 0.51 mmol) and wet cells (330 mg mL−1) at given pH values (pH 5.2 8.2 were prepared as potassium phosphate buffers and pH 4.2 was prepared as citrate/phosphate buffer, all at a buffer strength of 100 m m) at 28 °C for 6 h.