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230 µL p-nitrophenyl-β-d-galactopyranoside (p-NPG, 2 g/L dissolved in pH = 4.3 acetic acid sodium acetate buffer) was preheated at 37 °C for 5 min, and then immediately reacted with 20 µL the diluted supernatant of fermentation broth at 37 °C for 20 min.
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For DNA extraction, 7.5 ml of CTAB isolation buffer was preheated in a 30 ml centrifuge tube to 60°C in a water bath.
Both the 177LuCl3 solution (MDS Nordion, Ottawa, Canada) and the DOTA-BR96 conjugate in 0.25 M ammonium acetate buffer were preheated to 45°C for 10 min.
The supernatants were diluted with 100 mM sodium acetate buffer (pH5.5) to reach a volume of 1 mL, and the mixture was preheated at 37 °C for 5 min.
The fly ash was preheated at 400°C, and SiC was preheated at 800°C for 1 h to remove moisture and gases from the surface of the particulates.
Before the enzymes were added the mix was preheated at 65°C for 10 minutes.
The hypotonic solution was preheated at 37°C for 30min.
The reaction mixture was preheated at 95°C for 1 min.
The temperate was preheated at 95°C 5 min.
A fresh and hot solution of the sample was loaded in the cell that was preheated at 80 °C.
For the heat-inactivation experiments, the enzyme was preheated at 65 °C for 10 min prior to use.
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