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The buffer was poured into the tank covering the gel.
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The modified RIPA buffer was poured directly onto the scWestern surface.
Lysis buffer temperature was measured with a thermometer immediately before buffer was poured on the scWestern surface.
After incubation the buffer was poured off and the disks were washed with non-autoclaved MQ water.
Once on the ground, 200 mL of 7.2% phosphate buffered saline was poured into the bag, immersing the pad and promoting absorption of liquid.
Briefly, a solution containing 1.2% agarose and 0.4% human fibrinogen was prepared in 100 mM tris-HCl buffer (pH 7.4), and 10 mL was poured into a 100 × 15 mm Petri dish.
A mixture consisting of 1 ml of fibrinogen (5 mg/ml), 1 ml of 1% agarose solution and thrombin (1 unit) in 20 mM Tris HCl buffer, pH 7.6, containing 0.1 M NaCl was poured into each well.
The remaining buffer from the original 15 ml tube was poured into the 50 ml tube.
The resin/supernatant mixture was poured into a column and washed with a loading buffer of 25 mM Tris-Cl, pH 7.0, 0.5 M NaCl.
The mixture was poured into a column, which was washed with 4 column volumes of buffer A. Yeast RDL1 was eluted using a 120 mL linear gradient from 20 to 250 mM imidazole, which was formed with buffer A and buffer B [20 mM Tris-HCl (pH 8.0) containing 500 mM NaCl and 250 mM imidazole-HCl].
The resin or matrix was poured into a column, and the solAC protein was eluted by addition of lysis buffer containing 250 m m imidazole.
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