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Exact(10)
The enzymatic reaction mixture, comprising 1 ml of cellulase and 25 ml of 0.1 M acetate buffer, was placed in clean conical flasks.
Typically, 50 ml from SMJ with pH 7.0 ± 0.1 using MOPS buffer was placed in the beaker flasks with the soaking of 0.5 g of CUA, PACW, FW60, for 2 h with vigorous shaking at 30° C.
A slightly larger volume (430 μL) of dialysis buffer was placed in the reference compartment.
As a control, the same volume of M9 buffer was placed in adjacent wells.
For each sample, 1 mL of chilled buffer was placed in a small (5 cm diameter) Petri dish, on ice.
Each of the sera diluted 1 231 in sample buffer was placed in two antigen coated microtiter plate wells.
Similar(50)
One milliliter of 20 mM aqueous HAuCl4 and 4 mL of 100 mM aqueous TEA buffer were placed in 36 mL DI water under vigorous stirring at 25 °C.
One milliliter of 20 mM aqueous HAuCl4 and 4 mL of 100 mM aqueous PIPES-SS buffer were placed in 36 mL DI water under vigorous stirring at 150 °C for 10 min.
One milliliter of 20 mM aqueous HAuCl4 and 4 mL of 100 mM aqueous bis-tris methane buffer were placed in 36 mL DI water under vigorous stirring at 25 °C.
One milliliter of 20 mM aqueous HAuCl4 and 4 mL of 100 mM and aqueous bis-tris propane buffer were placed in 36 mL DI water under vigorous stirring at 100 °C for 5 min.
200 µl of 1 mM WT-ACD and 300 µl buffer were placed in the sample and reference cells, respectively.
More suggestions(18)
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