Sentence examples for buffer was pipetted into from inspiring English sources

Again, 500 µL of RPE buffer was pipetted into the tubes, and the columns were centrifuged for 15 seconds at 10,000×g and placed in new 2 ml catch tubes and spun at full speed for one minute to ensure that the membranes were dry.

A volume of 100 μl phosphate buffer was pipetted into the ring on the upper side (Fig.  1).

One 250 μg aliquot of actin was diluted to 1 mg/ml with 225 μl of ATP-supplemented general actin buffer, mixed to ensure complete re-suspension and left on ice for 30 min. 25 μl of actin polymerization buffer was pipetted into the actin protein and mixed well.

After washing, the standards, controls, serum samples, and incubation buffer were pipetted into the wells and further incubated at room temperature for 60 minutes.

To ensure that no beads have been residing in the deep well plate, 400 μl wash buffer is pipetted into the plate and a transfer to the filter plate is carried out as described above.

One milliliter of buffer was pipetted onto the column.

One hundred μL wash buffer 1 was pipetted into the purification column and centrifuged for 1 minute at 8,000 × g.

One hundred μL of wash buffer 2 (was was pipetted into the purification column and centrifuged for 1 min at 8,000 × g.

A black 96-well microplate was kept on ice, 120 µL of 112 nM fluorescein in 75 mM phosphate buffer (pH 7.4) was pipetted into each well, followed by 20 µL of standards, sample or blanks.

Next, 500 µL of buffer RPE (QIAGEN, RNeasy Minikit) was pipetted into each RNeasy column, and the tubes were centrifuged for 15 seconds at 10,000×g (gravity), decanting the flow-through afterwards.

The in vitro digestion assay (50 μl total volume) was set up as follows: 11.5 μM Aβ1 40 was pipetted into the reaction buffer (20 mM Tris, pH 7.4, 100 mM NaCl) containing 1 mM DTT and initiated by adding 0.8 μM of GST-PSA (the preparation's activity had been determined as described above).