Sentence examples for buffer was performed for from inspiring English sources
The incubation with 50 mM TRIS-HCl, pH 7.8, 10 mM CaCl2 buffer was performed for 19 h at 37°C.
Secondary antibody incubation (streptavidin-allophycocyanin) in flow buffer was performed for 30 minutes on ice.
Immediately after exsanguination, a bronchoalveolar lavage (using 40 mL sterile saline buffer) was performed for further biochemical investigations.
Incubation with primary antibodies (supplementary material Table S4) in dilution buffer was performed for 1 hour at room temperature.
Subsequent to the incubation stage, a buffer exchange with α-Syn-free buffer was performed for 6 s which removed unbound α-Syn.
Reverse transcription reactions using SuperScript II (Invitrogen) in the manufacturer's recommended buffer were performed for 50 minutes at 42°C using twice the recommended concentration of enzyme, 1 μl of Protector RNAse inhibitor (Roche) to avoid RNA degradation, 2 μl 5' primer (12 μM), 2 μl 10 mM DTT, and 1 μl 10 mM dNTPs.
For C. albipunctata embryos, the following modifications to the staining protocol apply: proteinase K treatment was carried out for 7 min at room temperature; post-hybridization washes: an additional wash of 10 min 2xSSC/hybridization buffer was performed before washing for 15 min with PBT/hybridization buffer; antibody incubation: embryos were incubated with anti-DIG for 2 hrs.
Migration in Tris glycine buffer was performed at 10 mA for 120 minutes and then at 20 mA for 60 minutes, using the Mini-Protean II® cell system from BioRad (CA, USA).
Electrophoresis in Tris borate ethylenediaminetetraacetic acid buffer was performed at 100 V for 1.5 h.
Electrophoresis in Tris-borate-EDTA buffer was performed at 100 V for 1.5 h.
