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Migration in Tris glycine buffer was performed at 10 mA for 120 minutes and then at 20 mA for 60 minutes, using the Mini-Protean II® cell system from BioRad (CA, USA).
Electrophoresis in Tris borate ethylenediaminetetraacetic acid buffer was performed at 100 V for 1.5 h.
Electrophoresis in Tris-borate-EDTA buffer was performed at 100 V for 1.5 h.
Electrophoresis in Tris-borate-EDTA buffer was performed at 120 V for 35 min.
Hybridization with the αP-dCTP-labeled probe at a final concentration of 10cpm/mL of hybridization buffer was performed at 42°C overnight.
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Incubations in deuterated buffer were performed at intervals from 10 s to 1 hr.
Careful rinses with several changes of TBS-0.3% tween buffer were performed at each step.
Incubations with primary and secondary antibodies, diluted in PBS buffer, were performed at 4°C overnight and at RT for 60 min, respectively.
Incubation with primary antibodies diluted in blocking buffer was performed overnight at room temperature (RT).
The incubation with 50 mM TRIS-HCl, pH 7.8, 10 mM CaCl2 buffer was performed for 19 h at 37°C.
Incubation with primary antibodies (supplementary material Table S4) in dilution buffer was performed for 1 hour at room temperature.
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