Sentence examples for buffer was performed as from inspiring English sources

Injecting peptide into buffer was performed as the blank titration.

Western blotting of proteins extracted in non-denaturing buffer was performed as previously described by our group [35].

The calcium assay (with the Fluo8-AM loading dye and wash buffer) was performed as described above.

Cell contact-dependent activation of monocytes by TCRζbright or TCRζdim T cells fixed in PBS containing 0.05% glutaraldehyde and neutralized with an equal volume of buffer containing 0.2 M glycine was performed as described.

The beads were collected by a brief centrifugation, washed (1× buffer-1, 2× buffer-2: both 30 mM Tris pH 7.5, 150 mM NaCl, 2 mM MgCl2, 2 mM CaCl2 with 1% (buffer-1) and 0.1% Triton X-100 (buffer-2)), resuspended in loading buffer and analyzed by immunoblotting, which was performed as described.

P buffer was added to the remaining resin in an equal volume and the washing procedure was performed as described.

The highest quality results were obtained when the entire LCM process was ≤30 min, i.e., from the point the slide was removed from dry ice and immersed in 75% EtOH until captured cells were placed in extraction buffer; thus, LCM should be performed as quickly as possible to minimize RNA degradation.

Buffer injection was performed as described previously [ 35].

After incubation in blocking buffer, antibody staining was performed as per standard procedures.

Total proteins were extracted from the protoplasts using the IP buffer, and immunoprecipitation was performed as described above.

Following the EdU detection reaction, larvae were incubated in blocking buffer and immunostaining was performed as described above.