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The activity of the purified MS70S, determined from the dipeptide (fMet-Leu) synthesis assay, was 50% (Fig. S2A), likely due to the fact the buffer was optimized for E. coli translation assays.
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The thickness of MgO buffer layers was optimized for structural and electrical properties of the epi-ZnO films.
Therefore, a new elution buffer containing phosphoric acid and acetonitrile was optimized for the enrichment prior to ESI MS (Imanishi et al. 2007).
Concentration and composition of the starting buffer were optimized as well.
Receiver gain was optimized for each temperature and sample.
The Ta was optimized for each gene and species combination.
The present system was optimized for a 2D reconstruction algorithm.
The assay conditions for Nluc (see Methods for buffer composition) were optimized for high luminescence intensity, sustained signal duration, and good working stability under typical laboratory conditions.
Obviously, such systems do not use the whole memory offered by the input/output buffers, which is optimized for a MIMO 4×4, but the channel emulator design is the same for all of them.
Concentrations of primer cocktail, PCR buffer, and MgCl2 were optimized for each individual gene.
Multicolor imaging is often required for biological experiments, but the buffers currently used are optimized for Alexa-647 or its close structural analogue Cy5.
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